Proper management and hygiene are the keys for the poultry industry, profit mainly depends on efficient
cleaning and disinfection. Disinfectants play an essential role in controlling pathogens in health care, animal production,
and food-related industries. The effectiveness of a disinfectant is mainly dependent on the active compound
chosen, its concentration, and the cleanliness of the surfaces to which it is applied. The purpose of this study was to
evaluate the effectiveness of some disinfectants (Prophyl 2000®, G7®, Pron-Tech®, Alkadox®, and Biodine®) at different
concentrations and contact times at 20, 40, 60, and 90 minutes against field isolated serotypes of E. coli, Pasteurella
multocida, Campylobacter jejuni, and Staphylococcus aureus from chicken and duck farms production at a titer of 3×106
CFU (Colony Forming Unit) /cm2 in the absence and presence of organic matter (O.M). The results showed that the
efficiency of disinfectants was significantly increase with high concentration and long contact time. The Prophyl 2000®
was the most powerful disinfectant against field pathogens, followed by G7®, Pron-Tech®, and Alkadox®, while Biodine
® was the weakest disinfectant at the same conditions. Moreover, the organic matter hindered bactericidal power
of many commercial disinfectants such as quaternary ammonium compounds (QUATS), halogen releasing agents
including iodine, chlorine and its compounds, but some were not affected, such as glutaraldehyde. Finally, the success
of the disinfection process in different poultry farms mainly depends on the selection of suitable disinfectants.