Staff Members - Benha University
Isolation, Characterization and Identification of Lactic Acid Bacteria as Probiotic
5 th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, 8 April 2021, Egypt (Conference Online) • 2021
Publication Information
Authors
El-Beksh, Amany E.; Abou-Aly, H.E.*; Zaghloul, R.E. ; El-Meihy, Rasha M.
Keywords
Not Available
Journal
5 th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, 8 April 2021, Egypt (Conference Online)
Publisher
Faculty of Agriculture, Benha University
Volume
Not Available
Issue
Not Available
Pages
311-322
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Lactic acid bacteria that grow as the adventitious microflora of foods or that are added to foods as cultures are
generally considered to be harmless or even an advantage for human health. The objective of this study was to
isolate, screen and identify lactic acid bacteria from different sources. As well, evaluate the more potent lactic
acid bacteria isolates for their probiotic characteristics. Ninety-six bacterial isolates were isolated from different
18 sources, 51 (53%) isolates were able to hydrolyze 0.5% bile salts while 28 isolates were able to hydrolyze 1%
bile salts. Also, only 11 isolates were able to tolerate 2% bile salts after 6h incubation periods. On the other hand,
lactic acid bacteria isolates were able to survive at pH 1.5 and 3.0 as well 8.0. Also, these isolates were potent for
cholesterol assimilation, NaCl tolerance, exopolysaccharides production, antioxidant activity. Lactic acid bacteria
isolate MS87 that exhibited excellent probiotic characteristics was identified using 16S rRNA sequence as
Pediococcus pentosaceus. This study led to suggest that environmental-derived lactic acid bacteria-strains could
be used as a potential source of probiotic.
generally considered to be harmless or even an advantage for human health. The objective of this study was to
isolate, screen and identify lactic acid bacteria from different sources. As well, evaluate the more potent lactic
acid bacteria isolates for their probiotic characteristics. Ninety-six bacterial isolates were isolated from different
18 sources, 51 (53%) isolates were able to hydrolyze 0.5% bile salts while 28 isolates were able to hydrolyze 1%
bile salts. Also, only 11 isolates were able to tolerate 2% bile salts after 6h incubation periods. On the other hand,
lactic acid bacteria isolates were able to survive at pH 1.5 and 3.0 as well 8.0. Also, these isolates were potent for
cholesterol assimilation, NaCl tolerance, exopolysaccharides production, antioxidant activity. Lactic acid bacteria
isolate MS87 that exhibited excellent probiotic characteristics was identified using 16S rRNA sequence as
Pediococcus pentosaceus. This study led to suggest that environmental-derived lactic acid bacteria-strains could
be used as a potential source of probiotic.