Tanninisoneofthemostcommonphytochemicalsecondaryphenolicmetabolites,whichiswidelydistributedin variousplantproduction systems. Dietaryintakeoftannin canexertdifferentactionson theimmunesystem.The aim of this study was to evaluate the influence of different concentrations on broiler chicken immunity. Three groups (n = 10 in each group) were evaluated: control group given a normal basal diet, high tannic acid (HT) group given high-dose tannic acid (30 g/kg diet) and low tannic acid (LT) group given low-dose tannic acid (0.5 g/kg diet) for 35 days. Feed intake and body weight were measured weekly. Cells were isolated from thymus, spleen and caecal tonsils at the end of the study. Lymphocyte subsets, monocytes phagocytosis and cytokine mRNA expression in spleen were evaluated. The results showed that HT group chickens had decreased daily gain, final body weight, daily feed intake and relative weights of lymphoid organs compared with other groups. In thymus, spleen and caecal tonsils, relative CD4+, CD8+, CD4+CD8+ and γδ+ cell populations in the HT group were significant decreased in comparison with those of other groups. The relative B cell population in the HT group was also significant decreased. Cytokine mRNA expression in spleen cells of the HT group was also significantly lower than that in other groups. Conversely, CD4+CD8+ subpopulations in spleen and caecal tonsils,γδ+ cells inspleen and B cells incaecal tonsils ofthe LT group were significantly higher than those of the control group. In addition, IFN-γ mRNA expression in the LT group was significantly higher than that of the control group. These results demonstrated that dietary intake of tannin had positive and negative effects on broiler chicken immunity in a dose-dependent manner.