Staff Members - Benha University
COMPARISON OF THREE DIAGNOSTIC TECHNIQUES FOR DETECTING BOVINE VIRUS DIARRHEA VIRUS (BVDV) IN BUFFY COAT SAMPLES
BVMJ • 2013
Publication Information
Authors
G.F. El-Bagoury, E.M. El-Nahas, Rbab. T.M. Hassanien and Nawal. M.A. Youssef
Keywords
BVDV, ACE, IFX, RRT-PCR
Journal
BVMJ
Publisher
Not Available
Volume
24
Issue
2
Pages
82-87
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Three diagnostic techniques were compared in terms of their ability to detect BVDV in Buffy coats.
Two hundred twenty seven BVDV Buffy coats were used. The techniques used were antigen captures
ELISA (ACE), virus isolation-immunoflourescent test (IFX), and one step real time reverse
transcription-polymerase chain reaction (RRT-PCR). Out of 227 Buffy coat samples, 35 (15.4%) were
found to be positive with RRT-PCR, 23 (10.1%) with ACE, and 20 (8.8%) with virus isolation-IFT.
The results obtained indicated that RRT-PCR is more suitable for the detection of BVDV in Buffy coats
when compared to other techniques, and ACE can be used for the routine diagnosis of BVDV infection
as an alternative technique to virus isolation-IFT
Two hundred twenty seven BVDV Buffy coats were used. The techniques used were antigen captures
ELISA (ACE), virus isolation-immunoflourescent test (IFX), and one step real time reverse
transcription-polymerase chain reaction (RRT-PCR). Out of 227 Buffy coat samples, 35 (15.4%) were
found to be positive with RRT-PCR, 23 (10.1%) with ACE, and 20 (8.8%) with virus isolation-IFT.
The results obtained indicated that RRT-PCR is more suitable for the detection of BVDV in Buffy coats
when compared to other techniques, and ACE can be used for the routine diagnosis of BVDV infection
as an alternative technique to virus isolation-IFT