Staff Members - Benha University
PREVALENCE OF ANTIBODIES TO BLUETONGUE VIRUS IN SMALL AND LARGE RUMINANTS AT DIFFERENT PROVINCES OF EGYPT
BVMJ • 2013
Publication Information
Authors
G.F. El-Bagoury, E.M. El-Nahas, Asmaa Moneer and Nawal. M.A. Youssef
Keywords
Bluetongue, cELISA, Small and large ruminants, Seroprevalence
Journal
BVMJ
Publisher
Not Available
Volume
24
Issue
1
Pages
100-105
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
A total of 618 serum samples of sheep, lambs (< 3 months), goats and cattle at five provinces in Egypt
were screened for qualitative analysis of the BTV antibodies using a commercial competitive ELISA
(cELISA) kit. The results showed an overall percentage of BTV positive sheep, lambs (< 3 months),
goats and cattle serum samples were 23.5%, 15%, 10.9% and 10.7% respectively. The results based
upon the serum samples showing optical density values more than 50 percent of the mean of negative
control were taken as positive for presence of BTV antibodies. A highest percentage of seropositivity
was found in Gharbia (20.5%), Alexandria (17.9%), Kafer-El sheikh (16.8%), Mounofia (9%) and
Bahera (9.6%) provinces. From examined sera all over the four seasons of the years 2011 and 2012
indicated the prevalence of BTV antibodies by 21.1% during autumn, 15.6% during winter, 11.1%
during summer and 10.2% during spring. In conclusion, BTV antibodies were widely prevalent in
sheep, lambs (< 3 months), goats and cattle in these provinces of Egypt and cELISA were found to be
sensitive and effective for screening of BTV group specific antibodies
were screened for qualitative analysis of the BTV antibodies using a commercial competitive ELISA
(cELISA) kit. The results showed an overall percentage of BTV positive sheep, lambs (< 3 months),
goats and cattle serum samples were 23.5%, 15%, 10.9% and 10.7% respectively. The results based
upon the serum samples showing optical density values more than 50 percent of the mean of negative
control were taken as positive for presence of BTV antibodies. A highest percentage of seropositivity
was found in Gharbia (20.5%), Alexandria (17.9%), Kafer-El sheikh (16.8%), Mounofia (9%) and
Bahera (9.6%) provinces. From examined sera all over the four seasons of the years 2011 and 2012
indicated the prevalence of BTV antibodies by 21.1% during autumn, 15.6% during winter, 11.1%
during summer and 10.2% during spring. In conclusion, BTV antibodies were widely prevalent in
sheep, lambs (< 3 months), goats and cattle in these provinces of Egypt and cELISA were found to be
sensitive and effective for screening of BTV group specific antibodies