Background: Women with hysterectomy suffer from many psychological and emotional problems that have a significant effect on their well beings and their quality of life. Aim: Study aimed to evaluate the effect of psycho-educational program on depressive symptoms, post-traumatic stress response and quality of life among women with Hysterectomy. Research design: A quasi-experimental design (one group pre/post test) was utilized to achieve the aim of the study. Setting: The study was conducted at obstetric and gynecologic department at Benha University Hospital, Benha city, Qualybia Governorate. Sample: A purposive sample of (40) women with hysterectomy. Tools: Tool (I) divided into two parts: - A structured Socio-demographic Interview Questionnaire sheet. Part two: Beck's Depression Inventory Scale, Tool (II) Post Traumatic Stress Scale and Tool (III) Quality of life scale. Results: Findings reflected that there were highly statistically significant reduction in the severity of the total levels of depressive symptoms, post-traumatic stress response and improvement in the total quality of life level post program implementation than before. Conclusion: The psycho educational program had a positive effect on reducing depressive symptoms, post-traumatic stress response and improving quality of life among women with Hysterectomy. Recommendations: Generalization of psycho-educational program for all women with hysterectomy in all hospitals to alleviate their depressive symptoms and post-traumatic stress and improve their quality of life.