Introduction: Atopic dermatitis is a multifactorial genetically based disease. Many candidate genes have now been determined to be associated with AD like the genes related to the structural abnormalities of the epidermis and inflammatory cytokines. Aim of the study was to assess interleukin 4 and 10 (IL-4 and IL-10) serum concentrations in AD patients and to correlate the values with the occurrence of genotypes of selected polymorphisms in genes encoding these cytokines. Material and methods: Forty AD patients (mean age 36.8 years) and 40 healthy controls were included in the study. Blood samples were analyzed for IL-4 and IL-10 concentrations with ELISA assay and genotyping for 590C/T IL-4 and 1082A/G IL-10 polymorphisms with PCR-RFLP. Results: The obtained results revealed statistically higher serum concentration of IL-4 and IL-10 in AD patients when compared to healthy controls (88.55 pg/ml vs. 74.52 pg/ml for IL-4 and 6.47pg/ml vs. 1.01 pg/ml for IL-10). Analyzing the association between level of the examined cytokines and genotype polymorphisms 590 C/T for the IL-4 gene and 1082 A/G for the IL-10 gene, we found a statistically higher IL-4 and IL-10 serum levels among carriers of the T and G alleles respectively both in AD and control groups. Conclusions: 590T and 1082G alleles of IL-4 and IL-10 genes may be associated with high levels of serum IL-4 and IL-10, which may increase the risk of AD.