Background
MicroRNAs play important role in post-transcriptional regulation of gene expression also in development and cellular processes., miR-221 is an oncogenic miRNA that plays important role in the initiation and progression of HCC, by affecting pro-oncogenic pathways thus miRNAs is a new class of targets for the development of miRNA-based therapeutic strategy. The mature miRNA act by competion with cellular target mRNAs moreover that leads to inhibition of the mRNA and expression of its direct targets
Aim of the work
The aim of this study is to investigate the effect of miRNA221 inhibitor on suppression of proliferation, induction of apoptosis in HCC cell line that may be a novel approach to be a therapy for HCC
Material & methods
HepG2 HCC cell line
MiR-221 inhibitor will be transfected in HCC cell line using ultrasound microbubbles
After transfection; Reverse transcription polymerase chain reaction (RT-QPCR) of CDKN1B/P27 genes and miR 221, MTT assay, flow cytometry for the effect of MiR-221 inhibitor on cell growth and apoptosis, caspase-3 activity measurment will be done
Results
Our data revealed that the delivery of anti-miR-221 by ultrasound microbubble contrast agents. In this study, cell proliferation inhibited and promoted the apoptosis of cells the expression of miR-221 decreased. Consequently, there was upregulation in the expression of CDKN1B/p27
Conclusion
The transfection of recombinant plasmids contained anti-miR-221 to suppress oncogenic effect of-miR-221 and cell proliferation was inhibited and cell apoptosis was induced in HepG2 cells. This indicated that effective role miRNA that may be novel gene therapy targets