| publication name | Synthesis and photochemistry of pH-sensitive GFP chromophore analogs |
|---|---|
| Authors | Alan R. Katritzkya, Corresponding author contact information, Megumi Yoshioka-Tarvera, Bahaa El-Dien M. El-Gendya, C. Dennis Hall |
| year | 2011 |
| keywords | |
| journal | |
| volume | Not Available |
| issue | Not Available |
| pages | Not Available |
| publisher | Not Available |
| Local/International | International |
| Paper Link | http://www.sciencedirect.com/science/article/pii/S0040403910023105 |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
GFP chromophore analogs (7a–e, 8, and 10a,b) containing 2-thienyl-, 5-methyl-2-furyl-, 2-pyrryl, and 6-methyl-2-pyridyl-groups were synthesized and their fluorescence spectra recorded in the pH range 1–7. NMR studies showed that protonation of 8 (2-thienyl system) inhibited photoisomerization (Z–E) about the exocyclic double bond but that protonation of 7c (E + Z) (2-pyrryl system) gave only 7cE. Fluorescence studies revealed enhancement of fluorescence intensity of 7c and 7b,e (furyl system) below pH 2.5 and gave a similar result for 10a (pyridyl system) below pH 6. Quantum yields at pH 1 were low, probably due to excited state proton transfer (ESPT).