The preservation of sperm is critical to the effectiveness of assisted reproductive technologies in bovine.
However, Plant extracts has lately appeared as a low-cost, it considers a natural source of chemicals for preserving,
also enhancing activity of sperm during different period storage. Current paper gives an apprise on the usage of garlic (Allium sativum) extract as an alternative additive for sperm preservation in buffalo bulls at animal production research institute, Sakha, Kafr El-Sheikh Governorate, Egypt. The experimental protocol was designed as four groups (Control, G1, G2 and G3). Buffalo-bulls’ semen were exposed to different concentrations of garlic extract (0, 3, 6 and 9%, respectively) to evaluate the semen quality parameters after cooling and freezing process. Data obtained showed that, progressive motility, live spermatozoa and acrosome integrity % were higher (P < 0.05) in G1, G2 and G3 with reduced mortality percentage than control group after cooling process. In addition, a significantly increased (P < 0.05) of pro-gressive motility, acrosome integrity and alive spermatozoa percentage in groups treated with different concentrations of garlic extract than control group. While both the abnormality and chromatin value were significantly lower (P < 0.05) in the groups treated with different concentrations of garlic extract than control group. Activity of glutathione peroxidase, activity of superoxide dismutase and alpha glucosidaseLysozyme elevated (P < 0.05) in G1, G2, G3 treated groups than control group. Different concentrations of garlic extract reduced the malondialdehyde than control. Finally, it can be concluded that garlic extract additive to extender would be useful to improve semen quality and antioxidant enzymes of buffalo-bulls spermatozoa after different processes (cooling and freezing) at variable time.