Congenital adrenal hyperplasia (CAH) is a rare disorder characterized by cortisol deficiency with/ without aldosterone deficiency, and androgen excess. The aim of this study was to evaluate the effect of the educational program on quality of life for mothers of children suffering from congenital adrenal hyperplasia. A quasi-experimental design was used. This study was carried out at Benha University Hospital, Benha Specialized Hospital for Children and Health Insurance Hospital affiliated to Ministry of Health. A convenient sample included all available mother`s (55) of children between the ages of birth and 16 years with congenital adrenal hyperplasia. Tools of data collection: Three tools were used; Tool I: A structured interviewing questionnaire, Tool II: Mothers' reported practice sheet, Tool III: Quality of Life Scale. Results was a highly statistically significant difference observed between the studied mothers total knowledge, quality of life and mothers reported practice regarding to congenital adrenal hyperplasia at pre and post of the educational program implementation P= (