Abstract: Background: Chronic pelvic pain (CPP) is one of the most common gyneacological disorders that negatively affect quality of woman s’ life and sexual health. Well understanding of CPP in the perspective of care to those women and involving those vulnerable women in caring themselves is highly required. Aim: the present study aimed to evaluate the effect of self-care instructional guideline on quality of life and sexual health among chronic pelvic pain women. Setting: the current study was conducted at the outpatient gyneacological clinic at Benha University Hospital. Research Design: A Quasi-experimental design was used. Sample: Purposive sample included 133 women diagnosed with chronic pelvic pain. Tools: Six tools were utilized for data collection; A structured interviewing questionnaire, knowledge assessment sheet, self-care practice assessment, pelvic pain severity assessment tool, quality of life and sexual health assessment tools. Results: the present study revealed a highly statistical significant improvement knowledge mean score increased from 11.69 to 36.13 at post intervention. In addition self-care practice was satisfactory improved, as practice mean score was increased from 12.89 to 29.59 at post intervention phase which subsequently affect improvement of both studied women quality of life and sexual health. Conclusion: Self-care instructional guideline is effectively improving quality of life and sexual health among women with chronic pelvic pain Recommendation: self-care instructional guideline regarding management of chronic pelvic pain should be considered for chronic pelvic pain women during treatment. Keywords: Self-care, instructional Guideline, quality of life, sexual health, chronic pelvic pain.