Staff Members - Benha University
Detection and genetic analysis of Bovine Ephemeral Fever Virus G gene in buffy coat samples from cattle at Qualyubia, Egypt 2017
Journal of Virological Sciences • 2019
Publication Information
Authors
El-Habbaa, A.S.; Mervat E. Radwan
Keywords
EBF virus, Cattle, sequences, nucleotide, amino acid
Journal
Journal of Virological Sciences
Publisher
Scope Med.
Volume
4
Issue
1
Pages
under press
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Background: Bovine Ephemeral Fever (BEF) is endemic in Egypt with repeated periodic outbreaks.
Objective: To investigate strain detection and genetic analysis of BEF virus among suspected cattle in August 2017, Qualyubia, Egypt.
Methods: Partial sequence was generated after detection by reverse transcription-polymerase chain reaction (RT-PCR) and subsequent gel purification of the amplified products of G gene of BEF virus.
Results: BEF virus was circulating in this region. Sequence analysis of G-gene of this Egyptian strain, comparing with sequences of BEF virus circulating globally and regained from GenBank, showed 100% nucleotide homology with BEF virus from Egypt 2014 but nucleotide and deduced amino acid substitution generates were showed with other BEF viruses that reduced this homology. Phylogeny showed that BEF viruses from Egypt 2014 and 2017 had close homology to BEF virus circulated in Israel during the same period suggesting that the virus was circulated in middle east.
Conclusion: These findings demonstrated the recent picture of BEF virus which incriminated for cattle infectivity and responsible for its persistence in the endemic areas. Such epidemiological data could guide the application of efficient control strategies of BEF virus in Egypt.
Objective: To investigate strain detection and genetic analysis of BEF virus among suspected cattle in August 2017, Qualyubia, Egypt.
Methods: Partial sequence was generated after detection by reverse transcription-polymerase chain reaction (RT-PCR) and subsequent gel purification of the amplified products of G gene of BEF virus.
Results: BEF virus was circulating in this region. Sequence analysis of G-gene of this Egyptian strain, comparing with sequences of BEF virus circulating globally and regained from GenBank, showed 100% nucleotide homology with BEF virus from Egypt 2014 but nucleotide and deduced amino acid substitution generates were showed with other BEF viruses that reduced this homology. Phylogeny showed that BEF viruses from Egypt 2014 and 2017 had close homology to BEF virus circulated in Israel during the same period suggesting that the virus was circulated in middle east.
Conclusion: These findings demonstrated the recent picture of BEF virus which incriminated for cattle infectivity and responsible for its persistence in the endemic areas. Such epidemiological data could guide the application of efficient control strategies of BEF virus in Egypt.