Bovine ephemeral fever (BEF) virus belonging to Ephemerovirus genus of the Rhabdoviridae family causes an economically important seasonal acute vector- borne disease. Routine vaccination, is one of the main controlling steps, particularly in countries where the disease is endemic. This study deal with comparative evaluation of humeral immune response of cattle using SNT and ELISA to three inactivated BEF vaccines. Inactivated BEF vaccine with Montanide ISA 206 (water-in-oil-in-water) adjuvant given in 2 doses one month apart gave high protective antibody titer lasted for 44 weeks’ post vaccination (WPV). The inactivated BEF vaccine with aluminum hydroxide gel adjuvant given in 2 doses one month apart gave satisfactory immune response lasted for 30 WPV then neutralizing antibodies waned rapidly. Live attenuated BEF vaccine that inactivated by saponin on time of vaccination give higher antibody titer which rise more rapidly lasted for 38 WPV only. It could be concluding that the inactivated BEF vaccine with Montanide ISA 206 adjuvant gave satisfactory higher and longer lasting antibody titer for 44 WPV and it was recommended to use this vaccine in endemic and non-endemic countries to control and prevent the disease especially before summer season