Vaccination programs using FMD virus and BEF virus vaccines were effective for the disease control but the repeated vaccination induces stress on animals with increased cost of vaccination for the farmer. The present study aimed to prepare an inactivated combined vaccine containing three FMD virus serotypes O, A, SAT2 and BEF antigens adjuvant with Montanide oil ISA 206. The prepared vaccine proved to be sterile and safe and induced humoral immune response in calves after single dose administration. Serum neutralizing antibody reached the protective titers at 3rd week post vaccination for FMD virus serotypes O, A and SAT2 and at 2nd week post vaccination for BEF virus. Serum neutralizing antibody maintained at the protective titers for up to 32 and 42 weeks post vaccination for FMD and BEF viruses, respectively without inducing any adverse reactions. In conclusion, animals could be safely vaccinated with combined inactivated FMD and BEF viruses vaccine without impairing the immune response against these antigens.