A locally prepared inactivated bivalent ISA 206 oil adjuvant FMD vaccine was tested for quality and then applied in calves from different governorates for evaluation of their humeral immune response under field conditions. Serum samples were collected from vaccinated calves for detection of specific FMD antibodies against type O1 and A using SNT and indirect ELISA. The prepared vaccine was able to induce detectable protective levels of specific antibodies for both serotypes (O1 and A) of FMD virus by the first month post vaccination and persisted till 8th month post vaccination as detected by SNT and ELISA. It is concluded that the prepared vaccine was highly potent and provide high and long immunity that can protect vaccinated calves under field conditions.