أعضاء هيئة التدريس - جامعة بنها
Isolation and Identification of Lumpy Skin Disease Virus from Naturally Infected Buffaloes at Kaluobia, Egypt
Global Veterinaria • 2011
معلومات البحث
المؤلفون
EM El-Nahas, AS El-Habbaa; GF El-bagoury; Mervat EI Radwan
الكلمات المفتاحية
Lumpy Skin Disease Buffaloes Isolation Egypt
المجلة العلمية
Global Veterinaria
الناشر
Global Veterinaria
المجلد
7
العدد
3
الصفحات
234-237
publication.type
International
رابط البحث
Not Available
المواد المرفقة
Not Available
الملخص
Lumpy skin disease virus (LSDV) was isolated from skin biopsies collected from clinically infected buffaloes at Kaluobia governorate. The virus was isolated on chorio-allantoic membrane (CAM) of embryonated chicken eggs (ECEs) and identified by agar gel precipitation test (AGPT) and indirect fluorescent antibody
technique (IFAT) using specific hyper immune serum against LSDV. Further investigations were carried out using electron microscopic (EM) examination and polymerase chain reaction (PCR) for attachment protein gene confirmed LSDV isolate with specific amplified product 192bp.
technique (IFAT) using specific hyper immune serum against LSDV. Further investigations were carried out using electron microscopic (EM) examination and polymerase chain reaction (PCR) for attachment protein gene confirmed LSDV isolate with specific amplified product 192bp.