Foot and Mouth Disease Virus (FMDv.) was diagnosed in oesophageal-pharyngeal fluid and tongue epithelial samples from clinically suspected cattle in some Egyptian governorates (Monofia, Kalubia, Beni-suef and Sharkia) between 2009–2010. The virus was successfully isolated on un-weaned mice and BHK-21cell line. The virus serotypes were identified using ELISA as FMD serotypes A and O. 43 out of 260 collected samples (17.2%) were positive to serotype (O) in comparison to 13 out of 260 (5.0 %) were positive to serotype (A). The identified FMDv. serotypes were confirmed by molecular identification of VP1 coding region on the viral genome with Reverse Transcription–Polymerase Chain Reaction (RT-PCR) using specific primers that gave 800 bp and 402 bp amplification product for FMDv. serotypes A and O, respectively.