The present study was carried out to evaluate the efficiency of three water disinfectant (chlorine, iodine and H2O2 ) against total bacterial count, total fungal count and fecal E.coli count in water samples collected from main source of Toukh poultry farms, Kaliobia governorate. Water samples were taken room poultry farm was supplied by underground water and examined total bacterial count, total fungal count, fecal E.coli count and organic matter and found that T.B.C is 500 cfu/ml, T.F.C. was 40 cfu/ ml and fecal E.Coli count was 320 cfu/ ml and organic matter was 1.3 mg/L. Different concentrations from previously mentioned disinfectants were prepared and applied at different exposure times and the results were as the following: Aquatabs Granules®: give 100 % reduction% of T.B.C. and fecal E.coli at concentration of 25 g/L at exposure time 30 min., while give100% reduction of T.F.C. at concentration of 30 g/L at exposure time 60 min.. Virkon-S®: give100% reduction % of T.B.C. and fecal E.coli at concentration of 1 g/L at exposure time 30 min., while give100% reduction of T.F.C. at concentration of 2 g/L at exposure time 60 min. Iodoblex®: give 100% reduction % of T.B.C and fecal E.coli at concentration of 10 ml/L at exposure time 30 min., while give 77.5 % reduction % of T.F.C. at concentration of 10 ml/L at exposure time 30 min.