Background: Eye care is a significant aspect in the management of comatosed patients. Aim: The study aimed to evaluate the effect of eye care guidelines on nurses' competence for comatosed patients. Design: Quasi- experimental research design was utilized in this study. Setting: The study was carried out in Intensive Care Unit at Benha University Hospital. Sample: Convenient sample (60) nurses. Tools: three tools were used, Tool (1): Self-administered questionnaire to assess nurses' knowledge regarding patient’s eye care, tool (2): Observational checklist to assess nurses' practice regarding eye care, Tool (3): Nurses’ attitude toward eye care. Results: The study revealed that 10% of studied nurses had good level of knowledge, 20% had satisfactory level of practices and 33.3% of the studied nurses had positive attitude score regarding eye care pre guidelines implementation, which improved immediately post guidelines implementation to became (83.3%, 86.7% and 93.3%), respectively with a highly statistically significant difference at (p≤0.001). Conclusion: Providing nursing guidelines has been shown to be effective on nurses' competence regarding eye care for comatosed patients. Recommendation: Continuous education and training of nurses regarding eye care for comatosed patients.