The link between p53 as a prognostic marker and O2-free radicals as
an aetiological factor for cancer bladder needs to be clarified. The current
work was therefore, carried on 50 male subjects. Their ages were ranged
from 38-80 years with the mean value 56.3 . They were 40 patients with
cancer bladder in different grades compared with 10 health male subjects
as controls. Our patients were classified histopathologically into 4 groups.
Each group comprised 10 patients. Venous blood samples were taken from
all patients , pre-operatively and another samples were taken after 21 days
, post operatively. Also tissue biopsies with safety margins were taken for
determination of malondialdehyde (MDA) and p53 protein.
The results of this work showed that; pre-operative and post
operative serum MDA and p53 were significantly increased in all grades
compared with the control group (p<O.O5) except post operative serum
MDA in grade III which showed non-significant difference .Comparative
study of post operative serum MDA and p53 versus pre-operative, the data
showed statistical significant decrease (p1<O.O5). However, tissue
biopsies homogenates showed that MDA was significantly decreased in
grade III and grade IV (p<O.O5) but non-significant in grade I and grade II
while p53 was significantly increased in all grades when compared with
those obtained from healthy tissues. Correlation study ; showed that ; preoperative
serum MDA had a significant positive correlation with serum
p53 in all grades of cancer (p<O.O5). Meanwhile, tissue MDA had a
significant positive correlation with tissue P53 in grade IV (p<O.O5).
We could conclude that; , free radicals, lipid peroxidation and over
expression of P53 were inter-related to each others. However, oxy radicals
cause genetic changes of P53 resulting in loss of programmed cell death
(apoptosis) which play a critical role in tumorgenesis. The rapid decline of
serum p53 after radical cystectomy seemed to be a useful prognostic
marker for follow up of patients with cancer bladder.