Two field trials were conducted during two successive seasons of 2018/2019 and 2019/2020 in Floriculture farm of Horticulture Department, Faculty of Agriculture, Benha University to study the effects of spraying some growth substances of kinetin, Salicylic acid, Calcium Thiosulfate and potassium Silicate and chemical fertilization (N.P.K) as well as their combinations on flowering growth and chemical composition of Matthiola incana plants to increase and improve the flowering quality of this plant to bed gardens ornamentation. Obtained results showed that the heaviest fresh and dry weights of inflorescence and flowering portion were recorded by 100 ppm kinetin-sprayed plants fertilized with NPK fertilization at the high level in both seasons. The tallest and the thickest flowering portion were recorded by 100 ppm kinetin-sprayed plants joined with NPK fertilization at the high level in the two seasons. The highest values of the number, diameter and fresh weight of floret were scored by 100 ppm kinetin-sprayed plants supplemented with NPK fertilization at the high level in both seasons. The combined treatment between kinetin at 100 ppm and chemical fertilization at the high level gave the highest flowering duration. The highest leaf total chlorophylls and total indoles contents were recorded by 100 ppm kinetin- sprayed plants supplemented with chemical fertilization at the high level in the two seasons. Conclusively, spraying Matthiola incann plants with kinetin at 100 ppm and fertilized them with chemical fertilizer at 100N: 200P; 200K kg/fed. produced the best flowering growth and quality of this plant