In the present study, the anti-oxidative effects of dietary Curcumin were evaluated in Nile tilapia (O. niloticus) challenged with cold temperature condition in floating cages system. Fish with an average weight of 22 ± 0.5 g were divided into four groups, and fed daily with free basal diet (control); 1, 2, and 3% curcumin (CUR) for a five-week period in floating cages system. Oxidative status and growth parameters were measured. Results indicated that Curcumin supplementation markedly enhanced antioxidative status which was noticed by enhanced superoxide dismutase, glutathione peroxidase, catalase and Lipid peroxidase activities. In addition to, noticed improvement in growth performance parameters including body mass gain, specific growth rate (SGR), condition factor (CF) and feed conversion ratio (FCR). The expression of related HSP70, IL-1β and CC5 were markedly up-regulated over the control. Conclusively, Curcumin diets markedly enhanced anti-oxidative status all over experimental period, proposing its usage as natural anti- cold stress supplement and thus could increase time spent in outdoor culture systems through improving tolerance to cold water temperature