Sixteen mature Bagrus bayad of both sexes (8 males and 8 females) were used to evaluate a trial for spawning induction using GnRHa combined with domperidone during spawning season. The experiment was carried out during May 2014. Fish were divided into 4 groups. Group 1 was control males and females Bagrus bayad , group 2 was females Bagrus bayad treated with dopamine antagonist (domperidone) alone, group 3 was females Bagrus bayad treated with domperidone and gonadotropin releasing hormone analogue (GnRHa), and group 4 was males Bagrus bayad treated with GnRHa and domperidone. GnRHa was used in the present study by dose of 20 ug/kg and dose of domperidone was 5 mg/kg. Results showed that no evidence for spawning induction. Histological examination showed structural changes in ovary of group 2 and group 3 in comparison to control ovary but there was no obvious change between structure of ovary of group 2 and group 3. In addition to, histological examination showed structural changes in the testis of group 4 in comparison to control testis. In both sex, histological examination did not identify any evidence of spawning. In conclusion, domperidone and/or GnRHa induced histological changes in the ovary and testis of Bagrus bayad but did not induce spawning. Further studies are needed with higher doses of GnRHa and/or carrying out the experiment during mid or late spawning season of Bagrus bayad .