This study was aimed to the optimum conditions for cellulose extraction from different agriculture wastes (rice straw, rice husk, wheat straw and sugar-cane bagasse) and the biological activity of sulfated cellulose. Sulfation of the isolated cellulose was achieved through adopting the method by using sulfuric acid as sulfation agent in presence of catalyst (D.C.C or 4-(Dimethylamino) pyridine. In vitro anticoagulation and fibrinolytic activities for sulfated cellulose were determined. The lysis percentages of the plasma clots at 37°C were recorded with each sample and compared to that of standard hemoclar. The fibrinolytic and anti-coagulation activities of different sulfated cellulose with and without catalyst were measured in order to verify the traditional uses of this modified cellulose. The FT-IR analysis was done to elucidating the product cellulose and sulfated cellulose. The highest substitution was in sulfated cellulose bagasse, rice straw, rice husk and wheat straw, respectively. It was concluded that addition of sulphate group into extracted cellulose enhance anticoagulation activities. The results indicated that the highest clotting time was found by using D.C.C catalyst at concentration of 500 μg mL-1. The lowest clotting time was reported without catalyst sulfated celluloses at same concentration. Determination of fibrinolytic effects of the modified sulfated cellulose revealed that they have good fibrinolytic activities. Sulfated bagasse cellulose in presence of catalyst whether D.C.C or 4-(Dimethylamino) pyridine exhibited fibrinolytic activities equivalent to the same amount of standard 'Hemoclar' preparation at concentration of 2000 μg mL-1. No cytotoxic effect of sulfated cellulose was observed on VERO cells. Results indicated that the LD50 of sulfated cellulose is more than 5000 mg kg-1 b.wt. and these compounds are practically non-toxic.