2-(N-Phthalimidomethyl)-4-chlorobenzylidene-1-[2-(2-mercapto-4-oxoquinazolin-3-yl)]-5-imidazole derivatives in addition to its sugar hydrazones were newly synthesized. The antimicrobial activity of the prepared compounds was evaluated against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Aspergillus niger and Candida albicans. The sugar hydrazones analogues were the highly active compounds.