A total of 50 samples of human ,foods were collected from different food samples and human contact from different foods shopes . Samples were examined microbiologically for the presence of Staphylococcus aureus was isolated from the samples in a ratio present 8 and identified by biochemical identification. S.aureus strains were tested for antimicrobial sensitivity and all strains showed a 100% resistant to ampicillin. The resistance to oxacillin, amoxicillin, trimethoprim, gentamicin and tetracycline was in a different ratio. However, All the strains were sensitive to levofloxacin and ciprofloxacin. Using PCR technique, amplification of some virulence gene as(tst,icaD, sea )and antibiotic resistance genes (mecA , blaz , vanA) was performed from the extracted DNA of S.aureus strains .All extracted DNA samples of S.aureus showed a positive results for mecA ,tst , blaz, icaD and sea genes