To perform biometric analysis of Egyptian indigenous donkeys´ reproductive organs using ultrasound and correlate the findings with serum hormonal levels, 25 apparent healthy jacks aged between 2-12 years were used. The ultrasonographic measurements of internal (bulbourethral, prostate, vesicular glands, colliculus seminalis and pelvic urethra) as well as external (testes, epididymis and scrotum) organs were measured over three-weeks period for each animal and for each structure. The scrotal length, width and thickness were measured with a caliper (mm). An impact of testicular size in normal donkeys was noticed on the measures (length and width) of the prostate gland, vesicular gland, ampulla ductus deference and colliculus seminalis. Testes measures were highly (p 100 mm3. Moreover, there was a close positive association between testicular volume and scrotal thickness (r=0.939, p