In recent years, different formulations such as Nano emulsions have been widely used for the
target delivery, and enhanced biological functions of pesticide combinations. In this work, contact
toxicity, biological aspects, haemocyte counts (THCs) and haemolymph volume against 4th instar
larvae of the black cutworm, Agrotis ipsilon was determined under laboratory conditions. Mortality
rates 4th instar larvae of A. ipsilon increased with raising concentration to 3% to reach (64.21%) for
Jojoba oil and (86.31%) for 0.1% Nano oil emulsion. The LC50 of crude oil was (2.853%), while that
for Nano emulsion was (0.381%). Also, Jojoba oil (crude and Nano) showed decrease in larval and
pupal weight. While, the resultant female’s showed, significant reduction in emergence and lower
number of deposited eggs /female. Data also, showed significant decrease in haemocyte counts
(THCs) and the volume of haemolymph compared to control. The obtained data confirmed that Nano
emulsion was more effective than Jojoba oil. Histological examination after feeding rats on seeds
wheat treated with LC50 of Nano Jojoba oil was carried out. Liver, kidney and stomach of the treated
rats were affected by treatment. Hence, the Nano emulsion slow-release-formulation may represent a
new category of bio pesticides and this should be considered in the integrated pest management
programs.