Silkworm, Bombyx mori L., is a monophagous insect that drives almost all required nutrients for its growth and development from mulberry leaf. Nutration plays a pivotal role in sericulture. It improves the growth, development, health, feed consumption and conversion of silkworm thereby improving the commerical traits. Application of the required nutrient in the required amount to mulberry plant is, therefore, very essential for the successful silkworm growth and cocoon production. The present investigation was carried out at the Department of Plant Protection, Faculty of Agriculture Moshtohor, Benha University. Chemical, Bio fertilizers and its mixture caused increase in traits of fresh cocoon weight, cocoon shell weight, cocoon shell ratio, total larval duration, hatchability, silk gland weight, filament length, and filament weight and filament size. While, application by bio fertilizer resulted better values for cocoon shell ratio, cocooning percentage, larval weight, total larval duration, silk gland length and filament length. Both chemical and bio fertilizes exhibited better values for pupal weight. Also, bio fertilizer acquired best values for pupation ratio and cocooning percentage. Control and chemical were earned highest values for fecundity and fertility traits. Mixture and bio fertilizers acquired best values for silk gland length.