Abstract The practices of safety measures by the clinical laboratory workers in hospitals are necessary for the
prevention of occupational hazards. The aim of this study was to evaluate the effect of safety training program for
laboratory workers' regarding prevention of occupational hazards. Design: A quasi-experimental design was used in
carrying out this study. Setting: The study was conducted at four governmental hospitals in Benha City: - are Benha
University Hospital, Benha Teaching Hospital, Health Insurance Hospital, and Fever Hospital which included
clinical laboratory. The sample of this study included convenient laboratory workers (100) whom are working
at four hospitals were participated in this study. Tools: 1- A structured interviewing questionnaire for the
socio-demographic characteristics of the laboratory workers, and their knowledge regarding occupational hazards,
2- An observational checklist for safe laboratory environment and practices of laboratory workers regarding
prevention of the occupational hazards. Results: Showed a significant positive effect of the program on knowledge
and practices of the laboratory workers (P < 0.001). This study concluded that the program has positive effect to
upgrade the laboratory workers' knowledge and improving their practices regarding prevention of occupational
hazards (P < 0.001). The study recommended that regulatory training program should be strengthened to ensure
basic lab safety practices in hospitals, and providing training courses for large number of hospitals laboratory
workers about prevention of occupational hazards and safety environmental condition.