Phytochemical analysis revealed that flavonoids, alkaloids, tritrepenes, steroid and resins components are present in flowers of Matricaria recutita. Flowers essential oil (EO) of M. recutita prepared by hydrodistillation was identified by (GC/Ms) a total of 37 compounds were identified in the plant oil. α-Bisabolol oxide (32.1%) was the principle constituent, followed by Camphene (9.8%), α-Pinene (9.2%), Isopropyl hexadecanoate (6.6%), Camphor (6.0), 1,8-Cineole (6.1%), Sabinene (3.9), α-Terpinene (3.2%). Essential oil was evaluated for its antifungal activity against some pathogenic fungi, R. solani and F. solani under laboratory condition, it was tested by food poisoned technique. Result indicated that the growth of fungi R. solani and F. solani at amount of 0.75, 0.375, 0.1875 and 0.0935 μL/mL compared with negative control (0 μL/mL) and positive control (Dimethomorph 0.5g/L). Essential oil at 0.75 μL/mL had good antifungal activity against the growth of R. solani and F. solani. R. solani was the more sensitive to essential oil M. recutita with the largest inhibition zone 73% followed by F. solani with inhibition zone 62% compared with negative control with inhibition zone 0.0% for R. solani and F. solani.by the use of by Dimethomorph 0.5g/L (fungicidal) for R. solani and F. solani it gaves the result of 76% and 71 % inhibition zone for R. solani and F. solani, respectively as a positive control.