In Egypt, babesiosis is an endemic parasitic disease of cattle and buffaloes caused by Babesia bovis and Babesia bigemina. The spectrum of disease manifestation is broad, ranging from a silent infection to fulminate malaria – like disease, resulting in severe hemolysis and occasionally death. Aim of work: The present study was done to detect rate of infection with babesiosis in suspected cattle and focusing on the zoonotic importance of the disease among humans in contact with diseased cattle and to determine the responsible vector in the studied areas. Material and Methods: The study was carried out on suspected 55 native bread cattle and 80 human cases in contact with them and 80 engorged female ticks collected from diseased cattle and surrounding environment. Clinical examinations, thin blood film stained with Giemsa and IFAT for IgG detection were done for cattle and humans and C.B.C was done for human cases. Collected ticks were identified using wing dissecting microscope and identification keys, haemolymph smears were stained and examined for babesiosis. Results: It was found that 36 (65.5%) of cattle were positive by Giemsa stained blood film, 38 (69.1%) by IFAT, 34 (61.8%) by both stained blood film and IFAT (IgG) and the total positivity was 40 (72.7%). In human cases 8 (10%) were positive by Giemsa stained blood film, 4 (5%) by IFAT for B. bigemina IgG, 3 (3.8%) by both blood film and IFAT (IgG) and total positivity was 9 (11.2%). There was no significant difference between infected and non infected human cases as regards erythrogram and platelet variables, and the positivity was higher in the age group ( 40y) it was found that 80% of isolated ticks showed kinetes of Babesia parasites. Conclusion: This study highlights the infection rate among selected cattle and humans in close contact. It is concluded that babesiosis is a zoonotic disease and IFAT is a reliable and valid technique for determining infection rate in humans and is recommended for screening blood donors to guard against transfusion transmitted babesiosis.