This work was conducted on 30 Fasciola positive patients of both sexes with age ranged between 5-59 yeas old, 20 patients infected with other parasites than Fasciola and 20 healthy individuals. Cases were subjected to clinical study, parasitological study, hematological study and serological study by IHAT. Clinically abdominal pain, anorexia and loss of weight were the major symptoms. Parasitologically, Fasciola infected patients were classified using Kato thick smear according to intensity of infection to mild infection group; 6 (20%), moderate infection group; 15 (50%) and heavy infection group; 9(30%). Hematologically; the mean hemoglobin value of fascioliasis patient was signifi cantly lower than that of control groups & the mean eosinophilic count was significantly higher than control groups. Serologically, IHAT proved 73% sensitivity and 92% sepecificity.
It was concluded that parasitological examination is the only definitive method for diagnosis of fascioliasis but its low sensitivity motivates the researchers to look for more sensi tive methods to avoid missing of infected cases. IHAT is an accepted tool as a screening assay for Fasciola infection.