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publication name Comparative study between ELISA IgG, IgM serological techniques & PCR in diagnosing & studying toxoplasmosis in Qualyobia Governorate, Egypt. J.Egypt.Soc.Parasitol., 32(2):475-486.
Authors A.F.EL Fakahany, A.Abd El Mabood,M.El Garhee,M Eraki
year 2002
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Abstract

ELISA IgG, 1gM antibodies and PCR for toxoplasmosis were performed on 55 women with complicated gestation and their babies. Besides. ELISA IgG and 1gM were applied on 27 uncomplicated gestation (mothers & babies) and 152 randomly selected individuals. Seropositivity to specific IgG antibodies was 36.4%. 59.2% and 57.9% and for Ig M was 27.3%. 7.4% and 10.5% in complicated gestation, uncomplicated gestation and random population respectively. PCR was positive in 20%, 50% and 60% of mothers with abortion, premature deliveries and deliveries of babies with congenital anomalies respectively. 55.5% and 40% were found seropositive for IgG from normal full term babies and abnormal babies. 13% of abnormal babies were 1gM positive and 46.6% were PCR positive from the same group. Introduction Toxoplasma gondii infection occurs worldwide, most infections are relatively asymptomatic. Severe disease manifestations occur in the developing fetus and in

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