Toxoplasma antibodies were detected in sera of 700 cases: 500 were collected randomly from individuals attending the outpatient clinics of Benha University hospitals of different ages and sexes and 200 from suspected inpatients from Obstetric, Ophthalmology, Internal Medicine and Surgery Departments. The indirect fluorescent antibody technique was performed. The results were correlated with the clinical picture, age, sex and residence. The general positivity rate as 14.57%: in the random group, 11.2% and in suspected group, 23% (P<0.001). The antibodies were more in females than in males in random group especially above 40 years old but with no obvious difference in rural and urban areas. In the supected group. Toxoplasma positivity were 24% in Obstetric cases: 15% in hepatosplenomegalic cases, 17.5% in prolonged fever cases. 28.57% in ophthalmic cases and 40% in lymphadenopathy cases.