Staff Members - Benha University
Effect of buffalo bull breeds on developmental competence and vitrification of invitro produced embryos
Asian Pacific Journal of Reproduction • 2018
Publication Information
Authors
Walid T.M. Soliman1, Al-Shimaa Al-H.H. El-Naby2, Karima Gh.M. Mahmoud3, Ahmed R.M. El-Khawagah2,
Mohamed M.M. Kandiel2, Mahmoud E.A. Abouel-Roos2, Alaa E.Abdel-Ghaffar2, AbdEl Salam I. El Azab
Keywords
In vitro fertilization
Buffalo breed
Embryo production
Vitrification
Journal
Asian Pacific Journal of Reproduction
Publisher
Karima Gh M. Mahmoud
Volume
7
Issue
6
Pages
270-273
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
To assess effect of buffalo bull breed on the development and cryotolerence of the
in vitro produced embryos. Methods: Three types of frozen semen were adopted; Egyptian,
Italian and cross-bred (Egyptian-Italian) breeds were used for in-vitro fertilization and
vitrification of their embryos. Oocytes were collected from buffalo ovaries and matured in
vitro for 24 h, then they were fertilized using the three semen breeds. The produced embryos
of morula and blastocysts were vitrified using ethylene glycol and dimethyl sulfoxide then
evaluated for their viability after warming. Results: The cleavage and blastocysts rates
significantly declined in oocytes fertilized by Egyptian (P
in vitro produced embryos. Methods: Three types of frozen semen were adopted; Egyptian,
Italian and cross-bred (Egyptian-Italian) breeds were used for in-vitro fertilization and
vitrification of their embryos. Oocytes were collected from buffalo ovaries and matured in
vitro for 24 h, then they were fertilized using the three semen breeds. The produced embryos
of morula and blastocysts were vitrified using ethylene glycol and dimethyl sulfoxide then
evaluated for their viability after warming. Results: The cleavage and blastocysts rates
significantly declined in oocytes fertilized by Egyptian (P