| publication name | Effectiveness of Using Brilliant Cresyl Blue Staining for Quality Evaluation and Developmental Competence of Immature and Mature Buffalo Oocytes |
|---|---|
| Authors | Al-shimaa Al-H. H. El-Naby1, Karima Gh.M. Mahmoud2,*, Youssef F. Ahmed2, Mahmoud E.A. Abouel-Roos1 and Gamal A.M. Sosa1 |
| year | 2016 |
| keywords | Buffalo, oocytes, BCB, embryo development |
| journal | GLOBAL JOURNAL OF ANIMAL SCIENTIFIC RESEARCH |
| volume | Not Available |
| issue | Not Available |
| pages | Not Available |
| publisher | Not Available |
| Local/International | International |
| Paper Link | Not Available |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
The present study aimed to evaluate the effect of brilliant cresyl blue (BCB) stain on quality and developmental competence of mature and immature buffalo oocytes. Oocytes were exposed to BCB stain diluted in mDPBS (DPBS with 0.4% BSA) for 60 min at 38.5ºC in a humidified air atmosphere before, after maturation. The cleavage and blastocyst rates were determined. Results showed the percentage of BCB+: BCBˉ in immature and mature buffalo oocytes was 54:45 and 70:30 respectively. In immature oocytes percentage of embryo cleavage rate was highly significantly (P