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Effectiveness of Using Brilliant Cresyl Blue Staining for Quality Evaluation and Developmental Competence of Immature and Mature Buffalo Oocytes
GLOBAL JOURNAL OF ANIMAL SCIENTIFIC RESEARCH • 2016
Publication Information
Authors
Al-shimaa Al-H. H. El-Naby1, Karima Gh.M. Mahmoud2,*, Youssef F. Ahmed2, Mahmoud E.A. Abouel-Roos1
and Gamal A.M. Sosa1
Keywords
Buffalo, oocytes, BCB, embryo development
Journal
GLOBAL JOURNAL OF ANIMAL SCIENTIFIC RESEARCH
Publisher
Not Available
Volume
Not Available
Issue
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Pages
Not Available
publication.type
International
Paper Link
Not Available
Supplementary Materials
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Abstract
The present study aimed to evaluate the effect of brilliant cresyl blue (BCB) stain
on quality and developmental competence of mature and immature buffalo
oocytes. Oocytes were exposed to BCB stain diluted in mDPBS (DPBS with
0.4% BSA) for 60 min at 38.5ºC in a humidified air atmosphere before, after
maturation. The cleavage and blastocyst rates were determined. Results showed
the percentage of BCB+: BCBˉ in immature and mature buffalo oocytes was
54:45 and 70:30 respectively. In immature oocytes percentage of embryo
cleavage rate was highly significantly (P
on quality and developmental competence of mature and immature buffalo
oocytes. Oocytes were exposed to BCB stain diluted in mDPBS (DPBS with
0.4% BSA) for 60 min at 38.5ºC in a humidified air atmosphere before, after
maturation. The cleavage and blastocyst rates were determined. Results showed
the percentage of BCB+: BCBˉ in immature and mature buffalo oocytes was
54:45 and 70:30 respectively. In immature oocytes percentage of embryo
cleavage rate was highly significantly (P