With the aim of preparation of diagnostic antigens for Candida albicans, three types of Candida subcellular components were extracted by mechanical and enzymatic extractions. Different methods of extraction frequently used in other studies were used to release proteins form intact cells of Candida albicans. Extract were obtained from intact cells by (i) sonication, (ii) treatment with Zymolyase and Trichoderma lysing enzymes (TLE), and (iii) sonication after enzymatic treatment. The extracts were separated with SDS- polyacrylamide gel electrophoresis. The antigenicity and the cross reactivity of these fractions were analyzed by using the ELISA technique. The results revealed that the three fractions are antigenic. The whole cell and the cell wall fractions were more antigenic than the membrane fraction. There were various degrees of cross reactivity between these fractions. Moreover, the specificity of these antigens was examined. The results revealed that these antigens were strict specific to C. albicans only. The results presented in this study could be considered as a step for development of diagnostic and immunotherapeutic products for detection and controlling the Candida albicans infections among animals.