Two types of vaccines were prepared. The first one is a bivalent clostridial vaccine (C. chauvoei and C. septicum). The second vaccine is a combined vaccine from C. chauvoei, C. septicum, P. multocida type A and D, and M. haemolytica. The effectiveness of both bivalent clostridial vaccine and pasteurella combined vaccine was investigated in guinea pigs, rabbits and mice. To the bacterial immunity of C. chauvoei component, the immune response of guinea pig was conducted. The results revealed that both types of vaccines (bivalent clostridial vaccine and combined one) gave 100% protection for guinea pigs, when challenged with up to 128 MLD spore suspension. The immune response of the prepared vaccines was investigated in rabbits based on the mean antitoxin titer for C. septicum. The results revealed that the both vaccines showed almost similar antibody titer. The immune response of mice was evaluated based on challenge test for estimation of bacterial immunogenicity of P. multocida. The results revealed that there was no difference in log protection in both vaccines.