Banner

Utility of tetrazolium blue for the colorimetric assay of tannins in tea.

• 1997
Back
Publication Information
Authors Alaa S. Amin.
Keywords Not Available
Journal Not Available
Publisher Not Available
Volume Not Available
Issue Not Available
Pages Not Available
publication.type Local
Paper Link Not Available
Supplementary Materials Not Available
Abstract
A simple, rapid and accurate colorimetric method is proposed for the determination of tannins, based on the reduction of tetrazolium blue in alkaline medium by tannins at 90 2 C for 15 min, leading to the formation of a highly coloured formazan derivative. Absorbance measurements were made at 527 nm and the calibration graph was linear for 0.2-9.0 g/ml of tannic acid. For more accurate analysis, the Ringbom optimum concentration range was found to be 0.5–8.2 g/ml. The relative standard deviation for the determination in a tea sample containing 7.55% tannins was 1.65%. Most of the ingredients commonly found in tea samples do not interfere with the determination. Several tea samples were analysed using the proposed method.