| publication name | Utility of tetrazolium blue for the colorimetric assay of tannins in tea. |
|---|---|
| Authors | Alaa S. Amin. |
| year | 1997 |
| keywords | |
| journal | |
| volume | Not Available |
| issue | Not Available |
| pages | Not Available |
| publisher | Not Available |
| Local/International | Local |
| Paper Link | Not Available |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
A simple, rapid and accurate colorimetric method is proposed for the determination of tannins, based on the reduction of tetrazolium blue in alkaline medium by tannins at 90 2 C for 15 min, leading to the formation of a highly coloured formazan derivative. Absorbance measurements were made at 527 nm and the calibration graph was linear for 0.2-9.0 g/ml of tannic acid. For more accurate analysis, the Ringbom optimum concentration range was found to be 0.5–8.2 g/ml. The relative standard deviation for the determination in a tea sample containing 7.55% tannins was 1.65%. Most of the ingredients commonly found in tea samples do not interfere with the determination. Several tea samples were analysed using the proposed method.