A selective, reproducible, and sensitive spectrophotometric method for uranyl determination was developed. The method is based on the selective complexation reaction of UO2‏ 2 ions with 2-(2-benzothiazolylazo) phenol(I), -4-methoxyphenol (II), and -3-hydroxyphenol(III) in the presence of sodium lauryl sulphate. The potentiality of the prepared reagents as new chromogenic ones for the determination of UO2‏ 2 ions are studied by extensive investigations of the optimum conditions favoring the formation of the colored complexes. The method allows the determination of UO2‏ 2 in the range of 0.05–1.60, 0.05–1.40, and 0.05–1.70 mgmL_1 at _max 585, 608, and 599nm using reagents I, II, and III, respectively. For more accurate results, Ringbom optimum concentration ranges were found to be 0.10–1.60 mgmL_1. The molar absorptivity, Sandell sensitivity, detection, and quantification limits are also evaluated. The interfering ions and their tolerance limits have been studied. The method was successfully applied to the determination of uranium from standard ores, geological samples, and synthetic matrices.