Purpose: This study evaluated the influence of human mesenchymal stem cells (h-MSCs) and/or vitamin E (vit E) on experimental hepatocellular carcinoma (HCC). Methods: Eighty five rats were divided into; group 1: twenty rats served as control group and group 2: sixty five rats received Diethyl Nitrosamine (DENA) followed by carbon tetra chloride (CCl4) to induce HCC. Five rats were scarified after 2 months to assess the induction of HCC. The other sixty rats were further subdivided into; group 2: fifteen HCC were untreated rats, group 3: fifteen HCC rats were treated by injection of 107 h-AMSCs, group 4: fifteen HCC rats were treated by vitamin E and group 5: fifteen HCC rats were treated by injection of 107 h-AMSCs and vitamin E. Histological, arginase-1 immunohistochemical examination were assessed and serum albumin and α-fetoprotein were estimated. Results: Histopathological examination of group 2 revealed presence of anaplastic carcinoma, focal nodular hyperplasia. Group 5 showed improvement in histopathological picture relative to group 3 and 4. Positive arginase-1 IHC reaction was observed in group 2 with decrease reactivity in all other groups. Conclusion: Combined treatment with h-AMSCs and vitamin E has tumor suppressive effect with ameliorating HCC histopathological picture and liver function.