Oxidative stress- associated defective sperm function is one of the commonest causes of male infertility, and until recently, it is difficult to evaluate and treat. Overcoming of such oxidative stress status (OSS) by suitable antioxidants sure may help those infertile patients. It is well established that Vitamin E is one of the major membrane protectant against reactive oxygen species (ROS). This study aimed to determine the effect of supplementation of Ham’s F10 media with Vitamin E and comparing the results with Baker’s Yeast (Saccharomyces cerevisiae) extraction, which according to EFSA (The European Food Safety Authority) has a QPS (Qualified Presumption of Safety) status, so it was selected to be our probiotic model. Statistically significant positive influence towards sperm reproductive potential has been revealed in both supplementations of Vitamin E and Baker’s Yeast extraction. The sperm vitality, motility percent, and grade of motility were significantly increased (P