Abstract: Male factors are known to contribute to infertility problem. Semen samples were obtained from 50 Leukocytospermic infertile men with (≥1x106 peroxidase positive WBC's/ml) attending Hawaa fertility center, Benha, Egypt. Semen samples were categorizedinto two infertile male cohorts, based on bacteriological culture, Group1: positive bacterial culture (n=31) and Group 2: negative bacterial culture (n=19) and the seminological parameters of the two infertile male cohorts were compared with healthy controls (n=50). Positive bacterial culture has been defined by pathologically significant bacterial growth (≥1x103 bacteria/ml). Statistically significant deteriorated volume (p