This study was carried out during 2018 and 2019 at the Research and Experiment Center, Fac. Agric., Benha Univ., Egypt. to analyses the impact of four different water regimes (two, four, six and eight irrigations supplies) on yield and biochemical compositions studies of four soybean varieties i.e. Crawford, Giza 111, Giza 35, and Giza 21 during both seasons. Four replications of a split-plot design were used to set up the treatments. In the main-plots, irrigation schedules were distributed at random, whereas soybean varieties were in the sub-plots. The 10.5 m2 sub-plot was made up of 5 ridges that were each 3.5 m long and 60 cm wide. Results revealed that, as compared to the control plants, all of the different treatments clarified important significant changes in all traits. Increasing the number of irrigations considerably boosted seed production when compared to the seasons' lowest number of irrigations. Meanwhile, chemical analysis significantly decreased by increasing number of irrigations, except for oil content. Soybean varieties were significantly varied in seed yield for both seasons of chemical analysis which was significant in the leaves and phosphorus percentage of seeds only except for proline content of leaves. Therefore, it was not sufficiently substantial for irrigation regimes and soybean seed production to interact.