Inflamatory Bowel Disease (IBD) is an idiopathic ailment, most likely including an invulnerable response of the body to its own particular intestinal tract. Separation amongst chrons disease (CD) and ulcerative colitis (UC) is still troublesome. Faecal neutrophil-derived proteins (mainly calprotectin and lactoferrin) assessment is receiving increasing attention as promising tools to differentiate organic bowel diseases and functional bowel diseases. The aim of this study is to evaluate and compare fecal calprotectin and lactoferrin as non invasive rapid test in comparison with colonoscopy invasive test in diagnosis and differentiating inflammatory bowel disease (Ulcerative Colitis / Crhon’sdisease ) from Intestinal Bowel Syndrome. Subjects and Methods: This study has been conducted on 30 patients divided as follows: Group I: 10 patients with IBD (4 males and 6 females), Group II: 10 patients with IBS(5 males and 5 females) (Diagnosis was based on Rome III criteria),Group III: 10 healthy volunteers (4 males and 6 females) as control group , they were recruited from the outpatient clinic of internal medicine in Banha University Hospital. Results: Cer Test Calprotectin + Lactoferrin combo card tests had a sensitivity, specificity, PPV and NPV of 90%, 100%, 100% and 90.91% respectively by CerTest Calprotectin + Lactoferrin combo card tests with AUC of 0.95. Conclusion: calprotectin-lactoferrin appear to be a clinically useful marker in differentiating IBD from IBS, moreover it can be used as an activity marker in IBD