Staff Members - Benha University
In vitro maturation (IVM) of camel oocytes using different maturation media.
Benha Veterinary Medical Journal • 2023
Publication Information
Authors
Shimaa A. Gwaid, Mohsen A. Agag, Alaa E. Abd El-Ghaffar, Ahmed R.M. Elkhawagah
Keywords
Dromedary camel, maturation media, oocyte maturation, oocyte retrieval
Journal
Benha Veterinary Medical Journal
Publisher
Benha Veterinary Medical Journal - ekb
Volume
44
Issue
1
Pages
30-33
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
The aim of the present study is to evaluate the in vitro maturation rate of camel oocytes using
different maturation media. Ovaries of 50 she-camels were collected during the breeding
season (from December to April). Ovaries were transferred to the lab in a thermal flask
containing warm saline solution within 2 hrs. After slaughtering. Oocytes were collected
using 2 techniques; aspiration of graafian follicles (2-5mm size) and slicing of the ovaries.
Depending on the morphological quality, the collected oocytes were selected and cultured in
different types of maturation media (GTL, HAM’S1, HAM’S 2, HAM’S 3) for 30-40 hours
at 38.5˚c, 5% CO2, and maximum humidity (95-96%). The obtained results revealed that the
slicing technique produced a higher oocyte recovery rate compared to the aspiration
technique. In addition, the maturation media composition mainly the FSH affects the in vitro
maturation rate of camel oocytes. It could be concluded that the type of oocyte maturation
media might be one factor that improve the rate of oocyte maturation.
different maturation media. Ovaries of 50 she-camels were collected during the breeding
season (from December to April). Ovaries were transferred to the lab in a thermal flask
containing warm saline solution within 2 hrs. After slaughtering. Oocytes were collected
using 2 techniques; aspiration of graafian follicles (2-5mm size) and slicing of the ovaries.
Depending on the morphological quality, the collected oocytes were selected and cultured in
different types of maturation media (GTL, HAM’S1, HAM’S 2, HAM’S 3) for 30-40 hours
at 38.5˚c, 5% CO2, and maximum humidity (95-96%). The obtained results revealed that the
slicing technique produced a higher oocyte recovery rate compared to the aspiration
technique. In addition, the maturation media composition mainly the FSH affects the in vitro
maturation rate of camel oocytes. It could be concluded that the type of oocyte maturation
media might be one factor that improve the rate of oocyte maturation.