The aim of the present study is to evaluate the in vitro maturation rate of camel oocytes using different maturation media. Ovaries of 50 she-camels were collected during the breeding season (from December to April). Ovaries were transferred to the lab in a thermal flask containing warm saline solution within 2 hrs. After slaughtering. Oocytes were collected using 2 techniques; aspiration of graafian follicles (2-5mm size) and slicing of the ovaries. Depending on the morphological quality, the collected oocytes were selected and cultured in different types of maturation media (GTL, HAM’S1, HAM’S 2, HAM’S 3) for 30-40 hours at 38.5˚c, 5% CO2, and maximum humidity (95-96%). The obtained results revealed that the slicing technique produced a higher oocyte recovery rate compared to the aspiration technique. In addition, the maturation media composition mainly the FSH affects the in vitro maturation rate of camel oocytes. It could be concluded that the type of oocyte maturation media might be one factor that improve the rate of oocyte maturation.