| publication name | Effect of epidermal growth factor on buffalo frozen spermatozoa biometry and metabolic activity |
|---|---|
| Authors | Mohamed MM Kandiel, Ahmed RM El-Khawagah, Karima Gh Mahmoud |
| year | 2017 |
| keywords | Buffalo Epidermal growth factor Lipid peroxidation Seminal enzymes Speriogram *Corresponding author: Mohamed MM Kandiel, Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, 13736, Toukh, Qaluobia, Egypt. Tel: (+2013) 2461-411 Fax: (+2013) 2460-640 E-mail: moahmed.kandil@fvtm.bu.edu.eg; kandiel75@hotmail.com 1. Introduction |
| journal | Asian Pacific Journal of Reproduction |
| volume | 6 |
| issue | 1 |
| pages | 43-48 |
| publisher | Not Available |
| Local/International | International |
| Paper Link | https://www.researchgate.net/publication/317279808_Effect_of_Epidermal_growth_factor_on_buffalo_frozen_spermatozoa_biometry_and_metabolic_activity?_sg=Bc5EcLFx9YnIwNp2BrufONytBy6yjzjL_ah6iHkHlaDoOa15O-B1Ac9lsD8ImWAqo_LNShIuMmC1I0GjYrTVyfkPgVj7sXC8WPUXLVg8.mtvc7-xVIEyekl0IPm-SVMc41V8bdQXcDgr4TsS-o0m2djC2WMfvUVk9WWb9nwIAscOdfzzTH0oKxiuotFRoWg |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
Objective: To assess the effects of epidermal growth factor (EGF) on the Egyptian buffalo bull frozen semen, EGF was incorporated at 0 (control), 50, 100, 200 and 400 ng/mL of extender (Bioxcell®). Methods: Semen features, spermatozoa biometry, total liberated amounts of enzymes (aspartate transaminase, alanine aminotransferase, lactate dehydrogenase, acid phosphatase and alkaline phosphatase) and lipid peroxidation markers (thiobarbituric acid reactive substances, malondialdehyde, glutathione peroxidase, nitric oxide, catalase (CAT) and superoxide dismutase (SOD)) were determined in the spermatozoa-free extracellular extender. Results: Spermatozoa membrane integrity significantly (P