Staff Members - Benha University
Effect of epidermal growth factor on buffalo frozen spermatozoa biometry and metabolic activity
Asian Pacific Journal of Reproduction • 2017
Publication Information
Authors
Mohamed MM Kandiel, Ahmed RM El-Khawagah, Karima Gh Mahmoud
Keywords
Buffalo
Epidermal growth factor
Lipid peroxidation
Seminal enzymes
Speriogram
*Corresponding author: Mohamed MM Kandiel, Department of Theriogenology,
Faculty of Veterinary Medicine, Benha University, 13736, Toukh, Qaluobia, Egypt.
Tel: (+2013) 2461-411
Fax: (+2013) 2460-640
E-mail: moahmed.kandil@fvtm.bu.edu.eg; kandiel75@hotmail.com
1. Introduction
Journal
Asian Pacific Journal of Reproduction
Publisher
Not Available
Volume
6
Issue
1
Pages
43-48
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Objective: To assess the effects of epidermal growth factor (EGF) on the Egyptian buffalo bull
frozen semen, EGF was incorporated at 0 (control), 50, 100, 200 and 400 ng/mL of extender
(Bioxcell®). Methods: Semen features, spermatozoa biometry, total liberated amounts of
enzymes (aspartate transaminase, alanine aminotransferase, lactate dehydrogenase, acid
phosphatase and alkaline phosphatase) and lipid peroxidation markers (thiobarbituric acid
reactive substances, malondialdehyde, glutathione peroxidase, nitric oxide, catalase (CAT) and
superoxide dismutase (SOD)) were determined in the spermatozoa-free extracellular extender.
Results: Spermatozoa membrane integrity significantly (P
frozen semen, EGF was incorporated at 0 (control), 50, 100, 200 and 400 ng/mL of extender
(Bioxcell®). Methods: Semen features, spermatozoa biometry, total liberated amounts of
enzymes (aspartate transaminase, alanine aminotransferase, lactate dehydrogenase, acid
phosphatase and alkaline phosphatase) and lipid peroxidation markers (thiobarbituric acid
reactive substances, malondialdehyde, glutathione peroxidase, nitric oxide, catalase (CAT) and
superoxide dismutase (SOD)) were determined in the spermatozoa-free extracellular extender.
Results: Spermatozoa membrane integrity significantly (P