| publication name | Evaluation of In Vitro Capacitation of Buffalo Frozen/Thawed Sperm by Different Techniques |
|---|---|
| Authors | B. Gasparrini · A.R. Elkhawagah · V. Longobardi · G.A. Sosa · G. Bifulco · M.E.A. Abouelroos · A.E. el-ghafar · G. Campanile · |
| year | 2015 |
| keywords | Buffalo Frozen Semen Capacitation Trypan Blue/Giemsa Chlortetracycline |
| journal | journal of reproduction and infertility |
| volume | 4 |
| issue | 3 |
| pages | 19-28 |
| publisher | Not Available |
| Local/International | International |
| Paper Link | https://www.researchgate.net/publication/284294232_Evaluation_of_In_Vitro_Capacitation_of_Buffalo_FrozenThawed_Sperm_by_Different_Techniques |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
This study aimed to determine the most reliable method to evaluate capacitation of buffalo frozen/thawed sperm. Frozen/thawed sperm were incubated in TALP medium in absence of capacitating agents (control) and in presence of 10 μg/ml heparin for 2 and 4 hrs. Capacitation was assessed by Trypan blue/Giemsa after lysophosphatidylecholine (LPC) exposure, chlortetracycline (CTC) fluorescence assay and immune-localization of tyrosine phosphorylated protein. Furthermore, we evaluated the effect of heparin on penetration, cleavage rates and kinetics of embryo development after heterologous IVF. The percentage of LPC-induced acrosome reacted (AR)-sperm increased (P